In this paper, mesterolone metabolic profiles were investigated carefully. Mesterolone was administered to one healthy male volunteer. Urinary extracts were analyzed by liquid chromatography quadruple time-of-flight mass spectrometry (LC-QTOFMS) for the first time. Liquid–liquid extraction was applied to processing urine samples, and dilute-shoot analyses of intact metabolites were also presented. In LC-QTOFMS analysis, chromatographic peaks for potential metabolites were hunt down by using the theoretical [M–H] − as target ions in full scan experiment, and their actual deprotonated ions were analyzed in targeted MS/MS mode. Ten metabolites including seven new sulfate and three glucuronide conjugates were found for mesterolone. Because of no useful fragment ion for structural elucidation, gas chromatography–mass spectrometry instrumentation was employed to obtain structural details of the trimethylsilylated phase I metabolite released after solvolysis. Thus, their potential structures were proposed particularly by a combined MS approach. All the metabolites were also evaluated in terms of how long they could be detected, and S1 (1 α -methyl-5 α -androst-3-one-17 β -sulfate) together with S2 (1 α -methyl-5 α -androst-17-one-3 β -sulfate) was detected up to 9 days after oral administration, which could be the new potential biomarkers for mesterolone misuse. Copyright © 2015 John Wiley & Sons, Ltd.
He said that he had never heard of a therapeutic-use exemption (TUE), which athletes who are taking certain substances would require to not be deemed in violation of anti-doping rules if they test positive for a prohibited substance. He said that he had never visited JADCO's website to make himself familiar with the responsibilities of an athlete as it relates to substances found within his or her system; he had never tried to educate himself on JADCO's rules; and he added that January 2017 was the first time in his five years as a professional athlete that he knew of the prohibited list regarding certain medications. While he admitted receiving pamphlets from JADCO, Livermore added that he had only read the ones that included the list of prohibited substances.